Subtracted cDNA Library

Syd Biotechnologies specializes in the construction of subtracted cDNA libraries enriched with differentially expressed genes by eliminating transcripts common to both experimental and control samples. This targeted approach enhances the discovery of unique or condition-specific gene expression.

Utilizing proprietary subtraction protocols and advanced PCR-based techniques, our team ensures thorough quality control at each stage of library preparation. The resulting libraries are highly enriched, accurately represent gene expression differences, and are ideal for downstream applications such as high-throughput sequencing and comparative transcriptomic analysis.

Service Highlights

• Choose any vector
• Subtraction is confirmed at various steps by northern, Q-RT PCR, colony hybridization and/or high throughput sequencing
• Mirror Orientation Selection (MOS) to eliminate false positive clones from SSH library
• Virtual northern blotting
• Receive two size fractionated libraries (0.4kb-2.0kb and 2.0 kb and up) for each sample
• 90-100% recombination efficiency
• >5 million primary clones
• Phage resistant competent cells used as host for propagation
• 250-500 µg endo-toxin free transfection grade DNA supplied with the library
• Semi-solid amplification procedure adapted for amplification to prevent biased clone representation