Syd Biotechnologies offers comprehensive yeast display library construction and screening services. Yeast display (also known as yeast surface display or YSD) is a powerful technique that enables the expression of proteins on the surface of yeast cells following proper folding and post-translational modifications within a eukaryotic system. Compared to phage display, yeast display offers significant advantages for the presentation of proteins and antibodies, as the eukaryotic environment of yeast allows for more accurate protein folding, modification, and translocation before surface display.
Libraries of human antibodies displayed on yeast are screened using flow cytometry, ensuring that the antibodies maintain conformational and functional properties closely resembling their native human forms.
Our scientists have over 20 years of experience and have developed a proprietary transformation protocol that consistently yields up to 2×10⁸ transformants per microgram of ligation mixture. This enables the generation of highly diverse and efficient libraries, making yeast display an excellent platform for high-affinity antibody discovery and affinity maturation.
Syd Biotechnologies utilises the yeast display system to monitor antibody maturation. Antibodies displayed on the surface of yeast cells are exposed to antigens under progressively stringent conditions. Yeast cells exhibiting strong antigen binding are isolated using Fluorescence-Activated Cell Sorting (FACS). The selected antibodies are subsequently purified through affinity chromatography.
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