Cattle OSM(Oncostatin M) ELISA Kit

Description

Catalogue Number:SPK7505
Name::Cattle OSM(Oncostatin M) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 2.9 pg/mL
Standard: 500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oncostatin M(Cattle OSM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Oncostatin M(Cattle OSM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oncostatin M(Cattle OSM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Oncostatin M(Cattle OSM) in the samples is then determined by comparing the OD of the samples to the standard curve.