Cattle PFN1(Profilin 1) ELISA Kit

Description

Catalogue Number:SPK8998
Name::Cattle PFN1(Profilin 1) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 32 pg/mL
Standard: 5000 pg/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Profilin 1(Cattle PFN1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Profilin 1(Cattle PFN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Profilin 1(Cattle PFN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Profilin 1(Cattle PFN1) in the samples is then determined by comparing the OD of the samples to the standard curve.