Cattle TGFb1(Transforming Growth Factor Beta 1) ELISA Kit

Description

Catalogue Number:SPK5467
Name::Cattle TGFb1(Transforming Growth Factor Beta 1) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 4.67 pg/mL
Standard: 1000 pg/mL
Sample Type: serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Transforming Growth Factor Beta 1(Cattle TGFb1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1(Cattle TGFb1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 1(Cattle TGFb1) in the samples is then determined by comparing the OD of the samples to the standard curve.