General Glu(Glutamic Acid) ELISA Kit

Description

Catalogue Number:SPK8234
Name::General Glu(Glutamic Acid) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 48.2 ng/mL
Standard: 10000 ng/mL
Sample Type: biological fluids
Assay Length: 2h
Research Area: Enzyme & Kinase;Endocrinology;Neuro science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glutamic Acid(Glu) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutamic Acid(Glu). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamic Acid(Glu) in the samples is then determined by comparing the OD of the samples to the standard curve.