General HVD3(25-Hydroxyvitamin D3) ELISA Kit

Description

Catalogue Number:SPK7890
Name::General HVD3(25-Hydroxyvitamin D3) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 0.91 ng/mL
Standard: 200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-Hydroxyvitamin D3(HVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3(HVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-Hydroxyvitamin D3(HVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.