General LTA4(Leukotriene A4) ELISA Kit

Description

Catalogue Number:SPK8074
Name::General LTA4(Leukotriene A4) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 46.3 pg/mL
Standard: 10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Infection immunity;Autoimmunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Leukotriene A4(LTA4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leukotriene A4(LTA4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leukotriene A4(LTA4) in the samples is then determined by comparing the OD of the samples to the standard curve.