General NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit

Description

Catalogue Number:SPK8020
Name::General NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 48.7 ng/mL
Standard: 10000 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;Tumor immunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Nicotinic Acid Adenine Dinucleotide Phosphate(NAADP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nicotinic Acid Adenine Dinucleotide Phosphate(NAADP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nicotinic Acid Adenine Dinucleotide Phosphate(NAADP) in the samples is then determined by comparing the OD of the samples to the standard curve.