General TMAO(Trimethylamine-N-oxide) ELISA Kit

Description

Catalogue Number:SPK8678
Name::General TMAO(Trimethylamine-N-oxide) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 4.93 ng/mL
Standard: 1000 ng/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Immunology;Innate Immunity;Complement;Alternative Pathway, Immunology;Innate Immunity;Complement;Other
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trimethylamine-N-oxide(TMAO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Trimethylamine-N-oxide(TMAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trimethylamine-N-oxide(TMAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Trimethylamine-N-oxide(TMAO) in the samples is then determined by comparing the OD of the samples to the standard curve.