General VD2(Vitamin D2) ELISA Kit

Description

Catalogue Number:SPK7870
Name::General VD2(Vitamin D2) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 0.53 ng/mL
Standard: 100 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Metabolic pathway;Neuro science;Nutrition metabolism;Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin D2(VD2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin D2(VD2). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D2(VD2) in the samples is then determined by comparing the OD of the samples to the standard curve.