Human 4-Hydroxynonenal (HNE) ELISA Kit

Description

Catalogue Number:SPK8372
Name::Human 4-Hydroxynonenal (HNE) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 19.1 pg/mL
Standard: 2500 pg/mL
Sample Type: Serum, plasma, tissue homogenates
Assay Length: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HNE(4-Hydroxynonenal ) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HNE(4-Hydroxynonenal ). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HNE(4-Hydroxynonenal ) in the samples is then determined by comparing the OD of the samples to the standard curve.