Human a1M(alpha 1 microglobulin) ELISA Kit

Description

Catalogue Number:SPK8606
Name::Human a1M(alpha 1 microglobulin) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.56 ng/mL
Standard: 100 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Infection immunity;Immune molecule;Kidney biomarker;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to alpha 1 microglobulin(a1M). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to alpha 1 microglobulin(a1M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain alpha 1 microglobulin(a1M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of alpha 1 microglobulin(a1M) in the samples is then determined by comparing the OD of the samples to the standard curve.