Human ACTL6A(Actin Like Protein 6A) ELISA Kit

Description

Catalogue Number:SPK5157
Name::Human ACTL6A(Actin Like Protein 6A) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Like Protein 6A(ACTL6A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Actin Like Protein 6A(ACTL6A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Like Protein 6A(ACTL6A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Like Protein 6A(ACTL6A) in the samples is then determined by comparing the OD of the samples to the standard curve.