Human ALOX12(Arachidonate-12-Lipoxygenase) ELISA Kit

Description

Catalogue Number:SPK4754
Name::Human ALOX12(Arachidonate-12-Lipoxygenase) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Neuro science;Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase(ALOX12). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase(ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase(ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-12-Lipoxygenase(ALOX12) in the samples is then determined by comparing the OD of the samples to the standard curve.