Human Anti-HPF4(Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit

Description

Catalogue Number:SPK8037
Name::Human Anti-HPF4(Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 2h, 30min
Research Area: Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Heparin/Platelet Factor 4 Antibody(Anti-HPF4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Heparin/Platelet Factor 4 Antibody(Anti-HPF4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Heparin/Platelet Factor 4 Antibody(Anti-HPF4) in the samples is then determined by comparing the OD of the samples to the standard curve.