Human Anti-IgA(Anti-Immunoglobulin A Antibody) ELISA Kit

Description

Catalogue Number:SPK8046
Name::Human Anti-IgA(Anti-Immunoglobulin A Antibody) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 2.36 ng/mL
Standard: 400 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Immune molecule;Hematology;Pulmonology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Immunoglobulin A Antibody(Anti-IgA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Immunoglobulin A Antibody(Anti-IgA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Immunoglobulin A Antibody(Anti-IgA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Immunoglobulin A Antibody(Anti-IgA) in the samples is then determined by comparing the OD of the samples to the standard curve.