Human Bid(BH3 Interacting Domain Death Agonist) ELISA Kit

Description

Catalogue Number:SPK1703
Name::Human Bid(BH3 Interacting Domain Death Agonist) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;Apoptosis;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BH3 Interacting Domain Death Agonist(Bid). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BH3 Interacting Domain Death Agonist(Bid). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BH3 Interacting Domain Death Agonist(Bid), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BH3 Interacting Domain Death Agonist(Bid) in the samples is then determined by comparing the OD of the samples to the standard curve.