Human carboxyhemoglobin,HbCO ELISA Kit

Description

Catalogue Number:SPK8401
Name::Human carboxyhemoglobin,HbCO ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 2.36 ng/mL
Standard: 2000 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research Area: Enzyme & Kinase;Cardiovascular biology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with carboxyhemoglobin,HbCO protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to carboxyhemoglobin,HbCO. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of carboxyhemoglobin,HbCO in the samples is then determined by comparing the OD of the samples to the standard curve.