Human CCR3(Chemokine C-C-Motif Receptor 3) ELISA Kit

Description

Catalogue Number:SPK2980
Name::Human CCR3(Chemokine C-C-Motif Receptor 3) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 13.7 pg/mL
Standard: 2000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 3(CCR3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 3(CCR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 3(CCR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 3(CCR3) in the samples is then determined by comparing the OD of the samples to the standard curve.