Human CTBP2(C-Terminal Binding Protein 2) ELISA Kit

Description

Catalogue Number:SPK3604
Name::Human CTBP2(C-Terminal Binding Protein 2) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Terminal Binding Protein 2(CTBP2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C-Terminal Binding Protein 2(CTBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Terminal Binding Protein 2(CTBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Terminal Binding Protein 2(CTBP2) in the samples is then determined by comparing the OD of the samples to the standard curve.