Human DGKA(Diacylglycerol kinase alpha) ELISA Kit

Description

Catalogue Number:SPK8837
Name::Human DGKA(Diacylglycerol kinase alpha) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.065 ng/mL
Standard: 10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diacylglycerol kinase alpha(DGKA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Diacylglycerol kinase alpha(DGKA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diacylglycerol kinase alpha(DGKA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diacylglycerol kinase alpha(DGKA) in the samples is then determined by comparing the OD of the samples to the standard curve.