Human ENPP2(Ectonucleotide Pyrophosphatase/Phosphodiesterase 2) ELISA Kit

Description

Catalogue Number:SPK3185
Name::Human ENPP2(Ectonucleotide Pyrophosphatase/Phosphodiesterase 2) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 43.21 pg/mL
Standard: 6000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 2(ENPP2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 2(ENPP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ectonucleotide Pyrophosphatase/Phosphodiesterase 2(ENPP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ectonucleotide Pyrophosphatase/Phosphodiesterase 2(ENPP2) in the samples is then determined by comparing the OD of the samples to the standard curve.