Human GSK3a(Glycogen Synthase Kinase 3 Alpha) ELISA Kit

Description

Catalogue Number:SPK1707
Name::Human GSK3a(Glycogen Synthase Kinase 3 Alpha) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.049 ng/mL
Standard: 10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;Endocrinology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Synthase Kinase 3 Alpha(GSK3a). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycogen Synthase Kinase 3 Alpha(GSK3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Synthase Kinase 3 Alpha(GSK3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycogen Synthase Kinase 3 Alpha(GSK3a) in the samples is then determined by comparing the OD of the samples to the standard curve.