Human LFA3(Lymphocyte Function Associated Antigen 3) ELISA Kit

Description

Catalogue Number:SPK2638
Name::Human LFA3(Lymphocyte Function Associated Antigen 3) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 5.8 pg/mL
Standard: 800 pg/mL
Sample Type: Serum, plasma, cell lysatesand other biological fluids.
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Function Associated Antigen 3(LFA3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lymphocyte Function Associated Antigen 3(LFA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Function Associated Antigen 3(LFA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Function Associated Antigen 3(LFA3) in the samples is then determined by comparing the OD of the samples to the standard curve.