Human P4Ha3(Prolyl-4-Hydroxylase Alpha Polypeptide III) ELISA Kit

Description

Catalogue Number:SPK3202
Name::Human P4Ha3(Prolyl-4-Hydroxylase Alpha Polypeptide III) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.066 ng/mL
Standard: 10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide III(P4Ha3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide III(P4Ha3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide III(P4Ha3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide III(P4Ha3) in the samples is then determined by comparing the OD of the samples to the standard curve.