Human PTPN6(Protein Tyrosine Phosphatase, Non Receptor Type 6) ELISA Kit

Description

Catalogue Number:SPK4435
Name::Human PTPN6(Protein Tyrosine Phosphatase, Non Receptor Type 6) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.105 ng/mL
Standard: 20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 6(PTPN6). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 6(PTPN6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase, Non Receptor Type 6(PTPN6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Tyrosine Phosphatase, Non Receptor Type 6(PTPN6) in the samples is then determined by comparing the OD of the samples to the standard curve.