Human TSHRAb(Thyroid Stimulating Hormone Receptor Antiboby) ELISA Kit

Description

Catalogue Number:SPK9540
Name::Human TSHRAb(Thyroid Stimulating Hormone Receptor Antiboby) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 0.85 mIU/mL
Standard: 100mIU/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Signal Transduction; Neuroscience
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroid Stimulating Hormone Receptor Antiboby(TSHRAb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroid Stimulating Hormone Receptor Antiboby(TSHRAb). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Stimulating Hormone Receptor Antiboby(TSHRAb) in the samples is then determined by comparing the OD of the samples to the standard curve.