Human ULBP2(UL16 Binding Brotein 2) ELISA Kit

Description

Catalogue Number:SPK6387
Name::Human ULBP2(UL16 Binding Brotein 2) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UL16 Binding Brotein 2(ULBP2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to UL16 Binding Brotein 2(ULBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UL16 Binding Brotein 2(ULBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UL16 Binding Brotein 2(ULBP2) in the samples is then determined by comparing the OD of the samples to the standard curve.