Mouse a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit

Description

Catalogue Number:SPK5551
Name::Mouse a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 0.94 μg/mL
Standard: 200 μg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Infection immunity;Immune molecule;Kidney biomarker;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-1-Microglobulin/Bikunin Precursor(a1M) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Microglobulin/Bikunin Precursor(a1M). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Microglobulin/Bikunin Precursor(a1M) in the samples is then determined by comparing the OD of the samples to the standard curve.