Rat ATP5b(ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide) ELISA Kit

Description

Catalogue Number:SPK7358
Name::Rat ATP5b(ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 1.21 ng/mL
Standard: 200 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide(ATP5b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide(ATP5b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide(ATP5b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide(ATP5b) in the samples is then determined by comparing the OD of the samples to the standard curve.