Rat MGMT(O-6-Methylguanine DNA Methyltransferase) ELISA Kit

Description

Catalogue Number:SPK7160
Name::Rat MGMT(O-6-Methylguanine DNA Methyltransferase) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 27 pg/mL
Standard: 5000 pg/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase(MGMT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase(MGMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-6-Methylguanine DNA Methyltransferase(MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of O-6-Methylguanine DNA Methyltransferase(MGMT) in the samples is then determined by comparing the OD of the samples to the standard curve.