Rat NUCB1(Nucleobindin 1) ELISA Kit

Description

Catalogue Number:SPK7021
Name::Rat NUCB1(Nucleobindin 1) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.063 ng/mL
Standard: 10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleobindin 1(NUCB1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nucleobindin 1(NUCB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleobindin 1(NUCB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleobindin 1(NUCB1) in the samples is then determined by comparing the OD of the samples to the standard curve.