Rat PEDF(Pigment Epithelium Derived Factor) ELISA Kit

Description

Catalogue Number:SPK6698
Name::Rat PEDF(Pigment Epithelium Derived Factor) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.062 ng/mL
Standard: 10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pigment Epithelium Derived Factor(PEDF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pigment Epithelium Derived Factor(PEDF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pigment Epithelium Derived Factor(PEDF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pigment Epithelium Derived Factor(PEDF) in the samples is then determined by comparing the OD of the samples to the standard curve.