Rat PI3K(Phosphotylinosital 3 kinase) ELISA Kit

Description

Catalogue Number:SPK8431
Name::Rat PI3K(Phosphotylinosital 3 kinase) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 3.9 pg/mL
Standard: 1000 pg/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphotylinosital 3 kinase(PI3K). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphotylinosital 3 kinase(PI3K). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphotylinosital 3 kinase(PI3K), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphotylinosital 3 kinase(PI3K) in the samples is then determined by comparing the OD of the samples to the standard curve.