Rat PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit

Description

Catalogue Number:SPK2409
Name::Rat PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 12.8 pg/mL
Standard: 2000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Bone metabolism;Rheumatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen II C-Terminal Propeptide(PIICP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen II C-Terminal Propeptide(PIICP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen II C-Terminal Propeptide(PIICP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen II C-Terminal Propeptide(PIICP) in the samples is then determined by comparing the OD of the samples to the standard curve.