Rat TRH(Thyrotropin Releasing Hormone) ELISA Kit

Description

Catalogue Number:SPK2607
Name::Rat TRH(Thyrotropin Releasing Hormone) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 2.43 pg/mL
Standard: 500 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Neuro science;Hepatology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(TRH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyrotropin Releasing Hormone(TRH) in the samples is then determined by comparing the OD of the samples to the standard curve.