Rat VD3(Vitamin D3) ELISA Kit

Description

Catalogue Number:SPK8950
Name::Rat VD3(Vitamin D3) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 2.14 ng/mL
Standard: 400 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin D3(VD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin D3(VD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D3(VD3) in the samples is then determined by comparing the OD of the samples to the standard curve.