Sheep vWF(Von Willebrand Factor) ELISA Kit

Description

Catalogue Number:SPK9355
Name::Sheep vWF(Von Willebrand Factor) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 4.11 ng/mL
Standard: 800 ng/mL
Sample Type: Plasma.
Assay Length: 2h
Research Area: Signal transduction;Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Von Willebrand Factor(vWF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Von Willebrand Factor(vWF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Willebrand Factor(vWF) in the samples is then determined by comparing the OD of the samples to the standard curve.