Avian 5-HT(5-Hydroxytryptamine) ELISA Kit

Description

Catalogue Number:SPK8694
Name::Avian 5-HT(5-Hydroxytryptamine) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 3.97 ng/mL
Standard: 900 ng/mL
Sample Type: serum and plasma
Assay Length: 2h
Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptamine(5-HT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine(5-HT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine(5-HT) in the samples is then determined by comparing the OD of the samples to the standard curve.