Cattle GPX1(Glutathione Peroxidase 1) ELISA Kit

Description

Catalogue Number:SPK5954
Name::Cattle GPX1(Glutathione Peroxidase 1) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 7.2 pg/mL
Standard: 1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1(Cattle GPX1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione Peroxidase 1(Cattle GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1(Cattle GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Peroxidase 1(Cattle GPX1) in the samples is then determined by comparing the OD of the samples to the standard curve.