Mouse S17aH(17-Alpha-Hydroxylase) ELISA Kit

Description

Catalogue Number:SPK7512
Name::Mouse S17aH(17-Alpha-Hydroxylase) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Alpha-Hydroxylase(S17aH). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-Alpha-Hydroxylase(S17aH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Alpha-Hydroxylase(S17aH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Alpha-Hydroxylase(S17aH) in the samples is then determined by comparing the OD of the samples to the standard curve.