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        • Shotgun Library
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Simian TIMP3(Tissue Inhibitors Of Metalloproteinase 3) ELISA Kit

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Home / Shop / ELISA / Simian TIMP3(Tissue Inhibitors Of Metalloproteinase 3) ELISA Kit
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Simian TIMP3(Tissue Inhibitors Of Metalloproteinase 3) ELISA Kit

$542.00

Simian TIMP3(Tissue Inhibitors Of Metalloproteinase 3) ELISA Kit

SKU: SPK7903 Category: ELISA
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Description

Catalogue Number:SPK7903
Name::Simian TIMP3(Tissue Inhibitors Of Metalloproteinase 3) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.112 ng/mL
Standard: 20 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Tumor immunity;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 3(TIMP3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 3(TIMP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Inhibitors Of Metalloproteinase 3(TIMP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tissue Inhibitors Of Metalloproteinase 3(TIMP3) in the samples is then determined by comparing the OD of the samples to the standard curve.

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